Abstract
The study involved seven species and cultivars of ornamental apple trees including Malus × floribunda, M. halliana, M. niedzwetzkyana, M. × purpurea, M. × purpurea ‘Ola’, M. × purpurea ‘Royalty’, and M. × purpurea ‘Selkirk’. The average value of increment, average length, and duration of shoots’ growth were determined to characterize the seasonal growth of Malus shoots. The percentages of rooted cuttings, callus formation without roots, and unviable cuttings were registered for each investigated taxon. Statistical analysis was performed in Microsoft Excel 2007 following Zaitsev (1990), and Atramentova & Utevskaya (2014).
The total duration of shoots growth of the studied species and varieties ranged from 72 (M. × purpurea ‘Ola’) to 118 days (M. niedzwetzkyana); annual shoots reached a length from 213.75 mm (M. × purpurea) to 448.75 mm (M. niedzwetzkyana); the average increment of shoots ranged from 3.90 mm (M. × purpurea ‘Ola’) to 14.70 mm (M. floribunda).
The rooting ability of Malus stem cuttings was limited to a reasonably short period of cutting procedure and depended on the application of biologically active substances, their concentrations, and complexes. The highest rooting rate (33.33 %) was observed in M. × purpurea ‘Ola’ after the treatment of its cuttings by 0.6 % indole-3-butyric acid (IBA) at the end of June. A slightly lower rooting rate (20.00 %) was observed in M. halliana cuttings treated by 0.4 % IBA and M. × purpurea ‘Selkirk’ cuttings treated by Podkorzen AB aqua at the end of June. Finally, 7.69 % of M. × floribunda cuttings were rooted with Podkorzen AB aqua at the beginning of July.
The prediction of the rooting ability of Malus stem cuttings was confirmed by a strong negative correlation between the percentage of rooted cuttings and the duration of shoots’ growth (r = –0.88). Hence, the percentage of rooted cuttings increases with decreasing duration of shoots growth, depending on the genotype.
References
Aleinikov, А. F., & Mineev, V. V. (2015). Method of diagnostics of softwood cuttings of sea-buckthorn for prediction of their rooting ability. Achievements of science and technology of AIC, 29(9), 77–79. (In Russian)
Amissah, J. N., Paolillo, D. J., & Bassuk, N. (2008). Adventitious root formation in stem cuttings of Quercus bicolor and Quercus macrocarpa and its relationship to stem anatomy. Journal of the American Society for Horticultural Science, 133(4), 479–486. https://doi.org/10.21273/JASHS.133.4.479
Atramentova, L. O., & Utevskaya, O. M. (2014). Statistics for biologists. NTMT. (In Ukrainian)
Aung, B., Gao, R., Gruber, M. Y., Yuan, Z. C., Sumarah, M., & Hannoufa, A. (2017). MsmiR156 affects global gene expression and promotes root regenerative capacity and nitrogen fixation activity in alfalfa. Transgenic research, 26(4), 541–557. https://doi.org/10.1007/s11248-017-0024-3
Bakhtaulova, A. S. (2020). Technology of Malus sieversii softwood cutting. IOP Conference Series: Earth and Environmental Science, 548(7), Article 082023. https://doi.org/10.1088/1755-1315/421/8/082023
Bakhtaulova, A. S., Oksikbayev, B. K., Kanagatov, Z. Z., & Dzhankuldukova, A. Z. (2015). Study of histostructure of ray parenchyma and rooting ability of Sievers apple (Malus sieversii) endemic species in green cutting. Oecologia Montana, 24(2), 70–73.
Besschetnova, N. N., Besschetnov, V. P., Kul’kova, A. V., & Mishukova, I. V. (2017). Starch content in shoot tissues of different spruce species (Picea A. Dietr.) in introduction. Forestry Journal, 4, 57–68. (In Russian). https://doi.org/10.17238/issn0536-1036.2017.4.57
Bilyk, О. (1993). Reproduction of woody plants by stem cuttings and grafting. Naukova Dumka. (In Russian)
Cubas, P. (2020). Plant seasonal growth: how perennial plants sense that winter is coming. Current Biology, 30(1), R21–R23. https://doi.org/10.1016/j.cub.2019.11.044
de Klerk, G. J., van der Krieken, W., & de Jong, J. C. (1999). Review the formation of adventitious roots: new concepts, new possibilities. In Vitro Cellular & Developmental Biology-Plant, 35(3), 189–199. https://doi.org/10.1007/s11627-999-0076-z
Doud, S.L., & Carlson, R. F. (1977). Effect of etiolation, stem anatomy and starch reserves on root initiation of layered Malus clones. Journal of the American Society for Horticultural Science, 102, 487–491.
Ermakov, B. S. (1981). Reproduction of woody plants and shrubs by the green cutting method. Shtiintsa. (In Russian)
Hansen, P. (1971). 14C-studies on apple trees. VII. The early seasonal growth in leaves, flowers and shoots as dependent upon current photosynthates and existing reserves. Physiologia Plantarum, 25(3), 469–473. https://doi.org/10.1111/j.1399-3054.1971.tb01475.x
Hartmann, H. T., Kester, D. E., Davies, F. T., & Robert, L. (2010). Propagation of ornamental trees, shrubs, and woody vines. In H. T. Hartmann, D. E. Kester, F. T. Davies & L. Robert. Hartmann and Kester’s plant propagation. Principles and practices. 8th edition. (pp. 774–839). Pearson
Ivanova, Z. Y. (1982). Biological bases and methods of vegetative propagation of woody plants by stem cuttings. Naukova Dumka. (In Russian).
Konopelko, A. V. (2020). Peculiarities of the reproductive biology of the genus Malus Mill. Journal of Native and Alien Plant Studies. 16, 96–111. (In Ukrainian). https://doi.org/10.37555/2707-3114.16.2020.219823
Molchanov, A. A., & Smirnov, V. V. (1967). Methodology for studying the increment of woody plants. Nauka. (In Russian)
Opalko, A. I., Yatsenko, A. O., Opalko, O. A., & Moiseychenko, N. V. (2004). Selection of fruit and vegetable crops. Naukoviy Svit. (In Ukrainian)
Opalko, A. I., & Opalko, O. A. (2019, February 25–27). Ex situ conservation strategy of woody plants depending on the features of their reproductive biology. In Proceedings of the international conferece “Plant conservation strategies of the botanic gardens and arboreta” (pp. 240–241). (In Ukrainian)
Opalko, О. А. (2003). Regenerative ability of cultivars, hybrid seedlings, clonal rootstocks and decorative forms of apple-trees in view of their economic properties. [PhD Dissertation, The Uman State Agrarian Academy]. (In Ukrainian).
Polikarpova, F. Y. (1990). Reproduction of fruit and berry crops by green cuttings. Agropromizdat. (In Russian)
Polikarpova, F. Y., & Pilyugina, V. V. (1991). Growing planting material by green cuttings. Rosagropromizdat. (In Russian)
POWO. (2021, May 28). Malus niedzwetzkyana Dieck. http://powo.science.kew.org/taxon/urn:lsid:ipni.org:names:726346-1
Schweingruber, F. H., & Börner, A. (2018). The plant stem: a microscopic aspect. Springer. https://doi.org/10.1007/978-3-319-73524-5
WFO. (2021, May 28). Malus niedzwetzkyana Dieck ex Koehne. http://www.worldfloraonline.org/taxon/wfo-0001012027
Xu, X., Li, X., Hu, X., Wu, T., Wang, Y., Xu, X., Zhang, X., & Han, Z. (2017). High miR156 expression is required for auxin-induced adventitious root formation via MxSPL26 independent of PINs and ARFs in Malus xiaojinensis. Frontiers in plant science, 8, Article 1059. https://doi.org/10.3389/fpls.2017.01059
Ye, B. B., Zhang, K., & Wang, J. W. (2020). The role of miR156 in rejuvenation in Arabidopsis thaliana. Journal of Integrative Plant Biology, 62(5), 550–555. https://doi.org/10.1111/jipb.12855
Yu, N., Niu, Q. W., Ng, K. H., & Chua, N. H. (2015). The role of miR156/SPLs modules in Arabidopsis lateral root development. The Plant Journal, 83(4), 673–685. https://doi.org/10.1111/tpj.12919
Zaitsev, G. N. (1990). Mathematics in experimental botany. Nauka. (In Russian)
Zhou, J., Wu, H., & Collet, G. F. (1992). Histological study of initiation and development in vitro of adventitious roots in minicuttings of apple rootstocks of M 26 and EMLA 9. Physiologia Plantarum, 84(3), 433–440. https://doi.org/10.1111/j.1399-3054.1992.tb04687.x

This work is licensed under a Creative Commons Attribution 4.0 International License.

