Keywords
secondary metabolites
Rosa damascena Mill.
in vitro culture
How to Cite
Abstract
The character of the diffusion of secondary metabolites of Rosa damascena Mill. into the culture medium was studied. The histochemical analysis of intact plants shoots and primary explants on contents of catechins and condensed tannins was carried out. According to the results, it was found that the synthesis of phenolic compounds actively occurs in the living tissues of the primary cortex and the medullary rays of annual shoots, where their concentration is 15–18 times higher than in the cells of sclerenchyma and phloem. The phenolic compounds were segregated actively – by primary explants of Lada variety, less actively by explants of Lan variety. It was shown that the intensity of the excretions of phenols from the stem into the culture medium had a spatial in homogeneity of the tissue that is topologically related to the arrangement of vegetative buds. It was found that the most active in this regard were the primary cortical zones directly located below buds. It was determined that the intensity of secondary metabolites excretions depended on the type of Rosa damascena, the radial dimension and the degree of explants woodiness.
References
Alekhno, H.D. and Vysotskyi, V.A. (1989), Klonalnoe mykrorazmnozhenyne roz [Clonal micropropagation roses]. Physiology and biochemistry of cultivated plants, N 18, pp. 489—493
Andreeva, V. A. (1988), Ferment peroksydaza: uchastye v zashchytnom mekhanyzme rastenyi [The enzyme peroxidase: participation in the defense mechanism of plants]. M.: Science, 128 р.
Dzhensen, U. (1965), Botanycheskaia hystokhymyia [Botanical histochemistry]. M.: World, 377p.
Zaprometov, M.N. (1993), Fenolnye soedynenyia. Rasprostranenye, metabolyzm y funktsyia v rastenyiakh [Phenolic compounds. Distribution, metabolism and function in plants]. M.: Science, 272 p.
Kalynyn, F.L., Kushnyr, H.P. and Sarnatskaia, V.V. (1992), Tekhnolohyia mykroklonalnoho razmno zhenyia rastenyi [Technology microclonal plant propagation]. K.: Scientific thought, 232 р.
Pylunskaia, O.A. (1999), Vvedenye v kulturu in vitro rozy efyromaslychnoi [Introduction to culture in vitro aromatic rose]. Proceedings of the Crimean State Agricultural University, pp. 88—97.
Pochynok, Kh.N. (1976), Metody byokhymycheskoho analyza rastenyi [Methods of biochemical analysis of plants]. K.: Scientific thought, 336 p.
Rubtsova, О.L. (2009), Rid Rosa L. v Ukraini: henofond, is toriia, napriamy doslidzhen, dosiahnennia ta perspektyvy [The genus Rosa L. in Ukraine: gene pool, history, research areas, developments and prospects]. K.: Phoenix, 375 p.
Rubtsova, О.L. and Chyzhankova, V.I. (2015), Selektsiia troiand: istoriia, dosiahnennia, suchasna stratehiia [Rose selection: history, achievements, modern strategy]. Plant Introduction, N 1, pp. 69—75. https://doi.org/10.5281/zenodo.2460813
Rubtsova, О.L. and Chyzhankova, V.I. (2016), Pidsumky introduktsii ta selektsii troiand u Natsionalnomu botanichnomu sadu im. M.M. Hryshka NAN Ukrainy [Results of introduction and breeding roses in M.M. Gryshko National Botanical Garden of the NAS of Ukraine]. Plant Introduction, N 2, pp. 12—17. https://doi.org/10.5281/zenodo.2353849
Makarevych, A.M., Shutova, A.H., Spyrydovych, E.V. and Reshetnykov E.V. (2010), Funktsyy y svoistva antotsyanov rastytelnoho syria [The functions and properties of anthocyanins vegetable raw materials]. Trudy BHU, N2, pp. 1—11.
Сharles, S.B., Imin, N. and Djordjevic, M.A. (2010), Flavonoids: new roles for old molecules. Journal of Integrative Plant Biology, N 52, pp. 98—111.
Charles, S.B. and Muday, G.K. (2004), The transparent testa mutation prevents flavonoid synthesis and alters auxin transport and the response of arabidopsis roots to gravity and light. The Plant Cell, N 16, pp. 1191—1205.
Ray, F.E. (2006), Esau’s Plant anatomy: meristems, cells, and tissues of the plant body: their structure, function, and development. New Jersey: Hoboken, pp. 473—501.
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