Keywords
hermetic conditions
photosynthetic pigments
assimilates allocation
mineral nutrients
amino acids
How to Cite
Abstract
The hermetic condition is the least studied factor associated with the spaceflights. Phalaenopsis pulcherrima is promising for space farming as it can be cultivated in small substrate blocks, and its photosynthetic apparatus is well adapted to elevated CO2 concentrations and temperatures.
Three-year-old meristematic P. pulcherrima plants were planted into plastic (acrylic) vessels filled with fibrous substrate. In control, vessels had an open top. The hermetic conditions were reached by sealing the vessels’ covers with a parafilm. Both control and hermetic vessels were placed in a plant growth chamber where test plants were cultivated under controlled conditions of air temperature, illumination, air humidity, and soil moisture. After 6 and 24 months of cultivation, the CO2 concentration in the hermetic and control vessels was measured, and the physiological characteristics of each test plant, such as the content of macro- and micronutrients, photosynthetic pigments, free amino acids, and content of labile carbohydrates (%) in the leaves of the test-plants were determined.
It was revealed that cultivation of P. pulcherrima in hermetic conditions affected its basic physiological processes such as photosynthesis, mineral nutrition, carbohydrates, and amino acid metabolisms. The effect size of this stress factor depended on the duration of exposition period. Long-term cultivation of P. pulcherrima under hermetic conditions promoted the accumulation of nonenzymatic antioxidants (viz. chlorophyll b, carotenoids, and amino acids), which contributed to the adaptation of this orchid species to oxidative stress caused by hermetic environment.
References
Brykov, V.A., Kovalenko, E.Y., & Ivanytska, B.A. (2018). Microcosm as a perspective model for biological experiments on nanosatellites. Space Science and Technology, 24(2), 55–59. https://doi.org/10.15407/knit2018.02.055
Cherevchenko, T.M., Zaimenko, N.V., & Kharytonova, I.P. (2000). Growth and the content of some assimilates in the leaves of orchids under long-term clinorotation. Ukrainian Botanical Journal, 56(1), 83–88. (In Ukrainian)
Hiscox, J.D., & Israelstam, C.F. (1979). A method for the extraction of chlorophyll from leaf tissue without maceration. Canadian Journal of Botany, 57(12), 1332–1334. https://doi.org/10.1139/b79-163
Khodadad, C.L., Hummerick, M.E., Spencer, L.E., Dixit, A.R., Richards, J.T., Romeyn, M.W., Smith, T.M., Wheeler, R.M., & Massa, G.D. (2020). Microbiological and nutritional analysis of lettuce crops grown on the International Space Station. Frontiers in Plant Science, 11, Article 199. https://doi.org/10.3389/fpls.2020.00199
Kiss, J.Z., Wolverton, C., Wyatt, S.E., Hasenstein, K.H., & van Loon, J. (2019). Comparison of microgravity analogs to spaceflight in studies of plant growth and development. Frontiers in Plant Science. 10, Article 1577. https://doi.org/10.3389/fpls.2019.01577
Manzano, A., Carnero-Diaz, E., Herranz, R., & Medina, F.J. (2022). Recent transcriptomic studies to elucidate the plant adaptive response to spaceflight and to simulated space environments. iScience, 25(8), Article 104687. https://doi.org/10.1016/j.isci.2022.104687
McElroy, J.S., & Kopsell, D.A. (2009). Physiological role of carotenoids and other antioxidants in plants and application to turfgrass stress management. New Zealand Journal of Crop and Horticultural Science, 37(4), 327–333. https://doi.org/10.1080/01140671.2009.9687587
Nguyen, M.T.P., Knowling, M., Tran, N.N., Burgess, A., Fisk, I., Watt, M., Escribà-Gelonch, M., This, H., Culton, J., & Hessel, V. (2023). Space farming: horticulture systems on spacecraft and outlook to planetary space exploration. Plant Physiology and Biochemistry, 194, 708–721. https://doi.org/10.1016/j.plaphy.2022.12.017
Oluwafemi, F.A., & Olubiyi, R.A. (2019). Investigation of corn seeds growth under simulated microgravity. Arid Zone Journal of Engineering, Technology & Environment. 15(SP.i2), 110–115. https://www.azojete.com.ng/index.php/azojete/article/view/17
Ovchinnikov, Y.A. (1974). Novel method of analysis of aminoacids, peptids and proteins. Publishing house “Mir”. (In Russian)
Serdyuk, M.E., Priss, O.P., Gaprindashvili, N.A., Zdorovtseva, L.M., Suharenko, O.I., & Ivanova, I.E. (2020). Research practicum. Part 1. Methods of fruit and vegetable and berry production research: textbook. Lux Publishing and Printing Center. (In Ukrainian)
Sharma, D., & Golam Kibria, B.M. (2013). On some test statistics for testing homogeneity of variances: a comparative study. Journal of Statistical Computation and Simulation, 83(10), 1944–1963. https://doi.org/10.1080/00949655.2012.675336
Song, S.J., Yun, D.L., Cho, A.R., & Kim, Y.J. (2019). Photosynthetic and growth response of phalaenopsis queen beer ‘mantefon’ to variable CO2 concentrations at different vegetative growth stages. Flower Research Journal, 27(1), 9–16. https://doi.org/10.11623/frj.2019.27.1.02
Wolff, S., Coelho, L., Zabrodina, M., & Brinckmann, E. (2013). Plant mineral nutrition, gas exchange and photosynthesis in space: A review. Advances in Space Research, 51(3), 465–475. https://doi.org/10.1016/j.asr.2012.09.024
Yi, Z., Cui, J., Fu, Y., & Liu, H. (2020). Effect of different light intensity on physiology, antioxidant capacity and photosynthetic characteristics on wheat seedlings under high CO2 concentration in a closed artificial ecosystem. Photosynthesis Research, 144, 23–34. https://doi.org/10.1007/s11120-020-00726-x
Zabel, P., Bamseya, M., Schubert, D., & Tajmar, M. (2016). Review and analysis of over 40 years of space plant growth systems. Life Sciences in Space Research, 10, 1–16. https://doi.org/10.1016/j.lssr.2016.06.004
Zaimenko, N.V. (1999). Effect of clinostating on physiological-biochemical processes in tropical orchids. Ukrainian Botanical Journal, 56(2), 174–179. (In Ukrainian)
Zaimenko, N.V., Ivanytska, B.O., Rositska, N.V., Didyk, N.P., Liu, D., Pyzyk, M., & Slaski, J. (2021). Physiological responses of orchids to prolonged clinorotation. Biosystems Diversity, 29(4), 367–373. https://doi.org/10.15421/012146
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