Keywords
Poaceae
antioxidant activity
2,2-diphenyl-1-picrylhydrazyl (DPPH)
ascorbic acid
carotene
How to Cite
Abstract
Objective – to estimate the antiradical scavenging ability of extracts of some plants of Brassicaceae Burnett and Poaceae Barnhart. species, to evaluate the total antioxidant activity of plants in the conditions of M.M. Gryshko National Botanical Garden of the NAS of Ukraine.
Material and methods. Plant material of this investigation – species, varieties and cultivars of Brassicaceaae and Poaceae families: Brassica campestris f. annua D.C. (BCA), B. campestris f. biennis D.C.× B. napus f. biennis D.C., cv. Innovatsiia (BCBNI), B. campestris f. biennis D.C. × B. rapa L. × B. napus f. biennis D.C. (BCBRBN), B. juncea (L.) Czern. (BJ), B. juncea (L.) Czern. f. biennis, cv. Annushka (BJA), B. napus f. biennis D.C., f. EROF-5 (BNE-5), B. rapa L. (BR), B. rapa subsp. rapifera Metzger (BRR), Raphanus sativus L. var. oleiformis Pers. (RSO), Miscanthus sacchariflorus (Maxim.) Benth., cv. Snihopad (MSS), M. sinensis Anderss. f. ESBMK-6 (MSE-6), Panicum virgatum L. f. PB (PVPB), P. virgatum L. f. PL (PVPL), Sorghum bicolor (L.) Moench. f. ETSSDF-11 (SBE-11), S. nigrum Roemer et Schultes f. ETSSCHF-1 (SNE-1), S. saccharatum (L.) Moench. cv. Medove (SSM), S. sudanense (Piper) Stapf (SS), S. technicum (Koern.) Bait. et Trab. (ST). The antioxidant activity of methanol and aqueous extracts based on the discoloration reaction on the solution of DPPH (2,2-diphenyl-1-picrylhydrazyl free radical) was determined according to Brandt-Williams et al. The determination of content of ascorbic acid was conducted according to V.P. Krishchenko, the content of carotene – according to B.P. Pleshkov.
Results. The antioxidant activity of methanol extracts of plants of Brassicaceae was registered in the range from 16.94±0.15 (BCA) to 36.91 ± 0.26 (BRR) % and aqueous extracts – from 26.53 ± 0.34 (BJ) to 65.85 ± 0.30 (RSO) %. This parameter for methanol extracts of Poaceae species was in range from 31.13 ± 0.32 (MSE-6) to 86.48 ± 0.49 (SNE-1) % and aqueous extracts – from 33.10 ± 0.47 (ST) to 83.14 ± 0.46 (PVPB) %. It was determined concentration of ascorbic acid from 118.67 ± 9.90 (BJA) to 566.61 ± 38.37 (BNE-5) mg% and carotene – from 0.48 ± 0.01 (BJA) to 3.11 ± 0.07 (BJ) mg% for Brassicaceae plants. Content of ascorbic acid in range from 11.80 ± 0.85 (PVPB) to 77.39 ± 1.29 (SS) mg% and carotene – from 0.05 ± 0.00 (PVPB) to 0.62 ± 0.02 (PVPL) mg% was identified for Poaceae plants.
Conclusions. This study showed that plant raw material of investigated plants is a valuable source of antioxidants and vitamins. Methanol and water extracts of plants of Poaceae had higher antiradical activity against DPPH radical than extracts of plants of Brassicaceae. The higher level of ascorbic acid and carotene was observed in extracts of plants of Brassicaceae. The minimal content of ascorbic acid and carotene found for BJA (Brassicaceae) and PVPB (Poaceae).
References
Vergun, O.M., Rakhmetov, D.B., Shymanska, O.V., Fishchenko, V.V., Druz, N.G. and Rakhmetova, S.O. (2017), Biohimichna harakterystyka syrovyny Camelina sativa (L.) Crantz [Biochemical characteristic of plant raw material of Camelina sativa (L.) Crantz]. Introdukciya Roslyn [Plant Introduction], vol. 74, N 2, pp. 80—89. https://doi.org/10.5281/zenodo.2300770
Krischenko, V.P. (1983), Metody ocenki kachestva rastitelnoy produkcii [Methods for evaluating of quality of plant production]. Moscow: Kolos, 192 p.
Pleshkov, B.P. (1985), Prakticum po biohimii rasteniy [Plant biochemistry workshop]. Moscow: Kolos, 256 p.
Acikgoz, F.E. (2016), Seasonal variations on quality parameters of Pak Choi (Brassica rapa L. subsp. chinensis L.). Advances in Crop Science and Technology, vol. 4, N 4. https://doi.org/10.4172/2329-8863.1000233
Martinez-Sanchez, A., Gil-Izquierdo, A., Gil, M.I. and Ferreres, F. (2008), A comparative study of flavonoid compounds, vitamin C, and antioxidant properties of baby leaf Brassicaceae species. Journal of Agricultural and Food Chemistry, vol. 56, pp. 2330—2340.
Prasad, M.S., Joshi, S., Narendra, K., Nadiya, S.K., Masthani, S.K., Phani, N.P. and Satya, A.K. (2015), A comparative study of phytochemical analyses and in vitro antimicrobial activity of three important vegetables from Brassicaceae family. International Journal of Research in Ayurveda and Pharmacy, vol. 6, N 6, pp. 767—772. https://doi.org/10.7897/2277-4343.066143
Agarwal, K. and Varma, R. (2014), Radical scavenging ability and biochemical screening of a common asian vegetable — Raphanus sativus L. International Journal Pharmaceutical Sciences Review and Research, vol. 27, N 1, article N. 23, pp. 127—134.
Anitha, T. and Divya dharsini, R. (2014), Studies on in vitro antioxidant properties of Brassica vegetables. International Journal of Pharmaceutical, Chemical and Biological Sciences, vol. 4, N 4, pp. 1061—1065.
Balcerek, M., Rak, I., Majtkowska, G. and Majt kowski, W. (2009), Antioxidant activity and total phenolic compounds in extracts of selected grasses (Poaceae). Herba Polonica, vol. 55, N 3, pp. 14—21.
Kucukboyci, N., Guvenc, A., Turan, N.N. and Aydin, A. (2012), Antioxidant activity total phenolic content of aqueous extract from Raphanus raphanistrum L. Turkey Journal of Pharmaceutical Sciences, vol. 9, N 1, pp. 93—100.
Soengas, P., Sotelo, T., Velasco, P. and Cartea, M.E. (2011), Antioxidant properties of Brassica vegetables. Functional Plant Science and Biotechnology, vol. 5, N 2, pp. 43—55.
Babu, R.and Savithramma, N.(2013), Phytochemical screening of underutilized species of Poaceae. Journal of Pharmacy Reaserch: BioMedRx, vol. 1, N 10, pp. 947—951.
Beltagy, A.M. (2014), Investigation of new antimicrobial and antioxidant activities of Brassica rapa L. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 6, N 6, pp. 84—88.
Fernandez-Leon, A.M., Lozano, M., Gonzales, D., Ayuso, M.C. and Fernandez-Leon, M.F. (2014), Bioactive compounds content and total antioxidant activity of two savoy cabbages. Czech Journal of Food Sciences, vol. 32, N 6, pp. 549—554.
Brand-Williams, W., Cuvelier, M.E. and Berset, C. (1995), Use of a free radical method to evaluate antioxidant activity. LWT — Food Science and Technology, vol. 28, N 1, pp. 25—30.
Rahman, M.S., Jahan, N., Khatun, M. and Rashid, M.A. (2015), Chemical and biological assays of Brassica rapa subsp. chinensis (L.) Hanelt. Bangladesh Journal of Botany, vol. 44, N 2, pp. 327—332.
Saha, P., Talukdar, A.D., Singh Ningthoujam, S., Choudhury, M.D., Lutfun, N., Nahar, D., Sarker, S.D. and Basar, N. (2015), Chemical composition, antimicrobial and antioxidant properties of seed oil plants of North-East India: A review. TANG (Humanitas Medicine), vol. 5, N 3, pp. 1—22. http://dx.doi.org/10.5667/tang.2015.0010
Devi, J.R. and Thangam, E.B. (2012), Studies on antioxidant and antimicrobial activities of purified sulforaphane from Brassica oleraceae var. rubra. Journal of Pharmacy Research, vol. 5, N 7, pp. 3582—3584.
Devi, P.S., Kumar, M.S. and Das, S.M. (2012), DNA damage protecting activity and free radical scavenging activity of anthocyanins from red sorghum (Sorghum bicolor) bran. Biotechnology Research International, Article ID 258787. http://dx.doi.org/10.1155/2012/258787
Walia, A., Malan, R., Saini, S., Saini, V. and Gupta, S. (2011), Hepatoprotective effects from the leaf extracts of Brassica juncea in CCl4 induced rat model. Der Pharmacia Sinica, vol. 2, N 4, pp. 274—285.
Gupta, V.K. and Sharma, S.K. (2006), Plants as natural antioxidants. Natural Product Radiance, vol. 5, N 4, pp. 326—334.
Kim, J.K., Baskar, T.B. and Park, S.U. (2016), Total phenolic and flavonoid contents and antioxidant activities of two Raphanus sativus L. cultivars (Cherry Belle and Valentine). Biosciences Biotechnology Research Asia, vol. 13, N 1, pp. 31—36. http://dx.doi.org/10.13005/bbra/1999
Kwak, Y., Lee, J. and Ju, J. (2016), Anti-cancer activities of Brassica juncea leaves in vitro. EXCLI Journal, vol.15, pp. 699—710. http://dx.doi.org/10.17179/excli2016-586
Marinova, G. and Batchvarov, V. (2011), Evaluation of the methods for determination of the free radical scavenging activity by DPPH. Bulgarian Journal of Agricultural Science, vol. 17, N 1, pp. 11—24.
Brosse, N., Dufour, A., Meng, X., Sun, Q. and Ragauskas, A. (2012), Miscanthus: a fast-growing crop for biofuels and chemicals production. Biofuels, Bioproducts and Biorefening. http://dx.doi.org/10.1002/bbb
Nahak, G., Suar, M. and Sahu, R.K. (2014), Antioxidant potential and nutritional values of vegetables: a review. Research Journal of Medicinal Plants, vol. 8, N 2, pp. 50—81. http://dx.doi.org/10.3923/rjmp.2014.50.81
Saeed, M. K., Anjum, S., Ahmad, I., Alim-un-Nisa, Ali, S., Zia, A. and Ali, S. (2012), Nutritional facts and free radical scavenging activity of turnip (Brassica rapa) from Pakistan. World Applied Sciences Journal, vol. 19, N 3, pp. 370—375. http://dx.doi.org/10.5829/idosi.wasj.2012.19.03.691
Pisoschi, A.M. and Negulescu, G.P. (2011), Methods for Total Antioxidant Activity Determination: A Review. Biochemistry and Analytical Biochemistry, vol. 1, N 1. http://dx.doi.org/10.4172/2161-1009.1000106
Podsedek, A. (2007), Natural antioxidants and antioxidant capacity of Brassica vegetables: a review. LWT — Food Science and Technology, vol. 40, pp. 1—11.
Prasad, M.P. (2014), Antimicrobial potential of Brassicaceae family against clinical isolates. International Journal of Pure and Applied Bioscience, vol. 2, N 2, pp. 158—162.
Woli, P., Paz, J.O., Lang, D.J., Baldwin, B.S. and Kiniry, J.R. (2012), Soil and variety effects on the energy and carbon balances of Switchgrass-derived ethanol. Journal of Sustainable Bioenergy Systems, vol. 2, pp. 65—74. http://dx.doi.org/10.4236/jsbs.2012.24010
Stahl, W. and Sies, H. (2003), Antioxidant activity of ca rotenoids. Molecular Aspects of Medicine, vol. 24, pp. 345—351.
Bors, M.D., Semeniuc, C.A., Socaci, S., Varva, L., Moldovan, O., Vlaic, R. and Tofana, M. (2015), Total phenolic content and antioxidant capacity of Radish as influenced by the variety and vegetative Stage. Bulletin UASVM Food Science and Technology, vol. 72, N 1, pp. 77—81. http://dx.doi.org/10.15835/buasvmcn-fst:11087
Singh, J., Upadhyay, A.K., Prasad, K., Bahadur, A. and Rai, M. (2007), Variability of carotenes, vitamin C, E and phenolics in Brassica vegetables. Journal of Food Composition and Analysis, vol. 20, pp. 106—112.
Warwick, S.I. (2011), Brassicaceae in Agriculture. In: Genetics and Genomics of the Brassicaceae. Plant Genetics and Genomics: Crops and Models, New York: Springer, pp. 33—65. http://dx.doi.org/10.1007/978-1-4419-7118-0_2
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