Keywords
antiradical activity
DPPH
methanolic extract
water extract
How to Cite
Abstract
Cosmos sulphureus is an adventitious species for Europe and Ukraine in particular. It originates from Central and South America, where it grows in tropical and subtropical climates. The climatic conditions of Kyiv are characterized as temperate, with an absolute temperature minimum of –32.2 °C, an absolute maximum of +39.9 °C, and average annual rainfall of 649 mm.
Plants were analyzed in the flowering phase, divided into inflorescences, leaves, stems, and roots, dried at +35 °C and then extracted with methanol and water. The determination of the antiradical activity was carried out according to a modified method using a DPPH (2,2-diphenyl-1-picrylhydrazyl) radical inhibition reaction. The highest antiradical activity was detected in inflorescences (59.60–81.81 % inhibition) and leaves (79.81–89.12 % inhibition). Stem extracts had an average level of inhibition (19.63–65.93 %), and root extracts showed only 2.54–39.46 % inhibition. Correlation analysis showed a strong relationship between leaves and stems (r = 0.84), leaves and roots (r = 0.81), and stems and roots (r = 0.91).
Extracts of C. sulphureus plants grown in temperate climate were found having a high antioxidant potential but lower than that reported for tropical and subtropical regions. It was found that higher intensity of coloration of marginal florets of the capitulum does not correlate with a higher antiradical activity. Methanolic and water extracts of inflorescences of the genotype CSCO-368812 with intensilly colored perianth inhibited only 59.60 % and 71.50 % of radicals, while similar extracts of the genotype CS-361294 with lighter florets inhibited 71.17 % and 81.81 % of radicals, respectively. Instead, there was a difference in antiradical activity depending on applied extractant. Methanolic extracts of vegetative organs (leaves, stems, roots) prevail over water extracts in terms of their antiradical activity. However, water extracts of inflorescences of both genotypes demonstrated higher level of antiradical activity.
References
Aguiar, G. P., Melo, N. I., Wakabayashi, K. A. L., Lopes, M. H. S., Mantovani, A. L. L., Dias, H. J., Fukui, M. J., Keles, L. C., Rodrigues, V., Groppo, M., Silva-Filho, A. A., Cunha, W. R., Magalhaes, L. G., & Crotti, A. E. M. (2013). Chemical composition and in vitro schistosomicidal activity of the essential oil from the flowers of Bidens sulphurea (Asteraceae). Natural Product Research, 27(10), 920–924. https://doi.org/10.1080/14786419.2012.671314
Andrushchenko, O., & Levon, V. (2021). The content of flavonoids in Cosmos sulphureus. Plant introduction, 89/90, 83–88. https://doi.org/10.46341/PI2021003
Botsaris, A. S. (2007). Plants used traditionally to treat malaria in Brazil: the archives of Flora Medicinal. Journal of Ethnobiology and Ethnomedicine, 3(18). https://doi.org/10.1186/1746-4269-3-18
Brand-Williams, W., Cuvelier, M. E., & Berset, C. (1995). Use of a free radical method to evaluate antioxidant activity. Lebensmittel Wissenschaften und Technologie, 28, 25–30.
Cavaiuolo, M., Cocetta, G., & Ferrante, A. (2013). The antioxidants changes in ornamental flowers during development and senescence. Antioxidants, 2, 132–155. https://doi.org/10.3390/antiox2030132
Cheng, S.-H., Khoo, H. E., Ismail, A., Abdul-Hamid, A., & Barakatun-Nisak, M. Y. (2016). Influence of extraction solvents on Cosmos caudatus leaf antioxidant properties. Iranian Journal of Science and Technology. Transactions A: Science, 40(1), 51–58. https://doi.org/10.1007/s40995-016-0007-x
Chensom, S., Okumura, H., & Mishima, T. (2019). Primary screening of antioxidant activity, total polyphenol content, carotenoid content, and nutritional composition of 13 edible flowers from Japan. Preventive Nutrition and Food Science, 24(2), 171–178. https://doi.org/10.3746/pnf.2019.24.2.171
Dian-Nashiela, F., Noriham, A., Nooraain, H., & Azizah, A. H. (2015). Antioxidant activity of herbal tea prepared from Cosmos caudatus leaves at different maturity stages. International Food Research Journal, 22(3), 1189–1194. http://ifrj.upm.edu.my/22%20(03)%202015/(43).pdf
Fernandes, L., Casal, S., Pereira, J.A., Saraiva, J.A., & Ramalhosa, E. (2017). Edible flowers: a review of the nutritional, antioxidant, antimicrobial properties and effects on human health. Journal of Food Composition and Analysis, 60, 38–50. https://doi.org/10.1016/j.jfca.2017.03.017
Jadav, K. M., & Gowda, K. N. N. (2017). Preliminary phytochemical analysis and in vitro antioxidant activity of Araucaria columnaris bark peel and Cosmos sulphureus flowers. International Journal of Current Pharmaceutical Research, 9(4), 96–99. https://doi.org/10.22159/ijcpr.2017v9i4.20967
Jang, I.-C., Park, J.-H., Park, E., Park, H.-R., & Lee, S.-C. (2008). Antioxidative and antigenotoxic activity of extracts from Cosmos (Cosmos bipinnatus) flowers. Plant Foods for Human Nutrition, 63, 205–210. https://doi.org/10.1007/s11130-008-0086-8
Kaisoon, O., Konczak, I., & Siriamornpun, S. (2012). Potential health enhancing properties of edible flowers from Thailand. Food Research International, 46(2), 563–571. https://doi.org/10.1016/j.foodres.2011.06.016
Kuzemko, A. A. (2013). Floral still life of the XVIII-XIX centuries as a source of information on the floral design of landscape gardens. Native and Alien Plant Sciences, 9, 38–50. (In Ukrainian) http://nbuv.gov.ua/UJRN/atiru_2013_9_7
Lakin, G. F. (1990). Biometrics. Vysshaya Shkola. (In Russian)
Lim, T. K. (2014). Edible medicinal and non-medicinal plants. Volume 8. Flowers. Springer. https://doi.org/10.1007/978-94-017-8748-2
Mediani, A., Abas, F., Khatib, A., & Tan, C. P. (2013). Cosmos caudatus as a potential source of polyphenolic compounds: optimisation of oven drying conditions and characterisation of its functional properties. Molecules, 18, 10452–10464. https://doi.org/10.3390/molecules180910452
Nash, D. L., & Williams, L. O. (1976). Flora of Guatemala. Fieldiana. Botany, 24(12): 1–603. https://archive.org/details/floraofguatemala2412nash/page/n1/mode/2up
Paniagua-Ibáñez, M., López-Caamal, A., Mussali-Galante, P., Sánchez-Salinas, E., Ortiz-Hernández, M. L., Ramirez-Rdríguez, R., & Tovar-Sánchez, E. (2015). Morphological variation of Cosmos bipinnatus (Asteraceae) and its relation to abiotic variables in central Mexico. Revista Chilena de Historia Natural, 88(14), 1–13. https://doi.org/10.1186/s40693-015-0044-4
Phuse, S. S., & Khan, Z. H. (2018). Estimation of free radical scavenging activity of Cosmos leaves extract. International Journal of Recent Scientific Research, 9(8), 28355–28358.
Prysedskyi, Y. (2014). Influence of soil pollution by fluorides and sulfites on grown parameters of some flowery-decorative plants. News of Dnipropetrovsk State Agrarian and Economic University, 33(1), 115–119. (In Ukrainian)
Ram, B., Mandlik, M., & Kumar, K. (2013). Antimicrobial activity of Cosmos sulphureus flowers around Pune. International Journal of Pharmaceutical Research and Development, 5(9), 27–31. https://innovareacademics.in/journals/index.php/ijcpr/article/view/20967/11942
Saleem, M., Ali, H. A., Akhtar, M. F., Saleem, U., Saleem, A., & Irshad, I. (2019). Chemical characterisation and hepatoprotective potential of Cosmos sulphureus Cav. and Cosmos bipinnatus Cav. Natural Product Research, 33(6), 897–900. https://doi.org/10.1080/14786419.2017.1413557
Schlangen, K., Miosic, S., Thill, J., & Halbwirth, H. (2010). Cloning, functional expression, and characterization of a chalcone 3-hydroxylase from Cosmos sulphureus. Journal of Experimental Botany, 61(12), 3451–3459. https://doi.org/10.1093/jxb/erq169
Vargas-Amado, G., Castro-Castro, A., Harker, M., Villaseñor, J. L., Ortiz, E., & Rodríguez, A. (2013). Distribucion geografica y riquera del genero Cosmos (Asteraceae: Coreopsideae). Revista Mexicana de Biodiversidad, 84, 536–555. https://doi.org/10.7550/rmb.31481
Vrublevska, O. O., & Katerusha, G. P. (2012). Climate in Ukraine and applied aspects of its implication. Tutorial. TES Private Enterprise. (In Ukrainian). http://eprints.library.odeku.edu.ua/378/1/VrublevskayaAA_Klimat_Ukrainy_%20ta_prikladni%20aspekty_igo_vykorystanya_2012.pdf
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