Introduction of Stevia rebaudiana (Bert.) Bertoni plants in M.M. Gryshko National Botanical Garden of the NAS of Ukraine
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Keywords

introduction
Stevia rebaudianа (Bert.) Bertoni
steviosides
flavonoids
ascorbic acid
long-term deposit
drought tolerance

How to Cite

Rakhmetov, D., Levchyk, N., Shpak, L., Hrakhov, V., Boiko, O., Lyubinska, A., Rakhmetova, S., & Zavhorodniy, V. (2016). Introduction of Stevia rebaudiana (Bert.) Bertoni plants in M.M. Gryshko National Botanical Garden of the NAS of Ukraine. Plant Introduction, 69, 3-17. https://doi.org/10.5281/zenodo.2333352

Abstract

The results of long-term studies of Stevia rebaudiana (Bert.) Bertoni are presented, viz. plant morphology parameters, propagation, cultivation, constituents. Stevia is characterized by significant food and medicinal properties and is promising introduced species in Ukraine. In M.M. Gryshko National Botanical Garden the valuable gene pool is accumulated which has numbered more than 20 forms. The nutritional media and environment needs for introduction to the culture in vitro and long-term storage of plant and also their regeneration from leaf explants. The content of antioxidants (flavonoids and ascorbic acid) in stevia raw material has been evaluated. The leaves of Stevia rebaudiana have been accumulated the diterpeneglycosides up to approximately 6–7 % (to dry weight). This is dynamic indicator that depends on Stevia form and cultivation conditions. Maximal accumulation of steviosides was observed in open ground plants in budding-bloo ming phases. Stevia rebaudiana plants regardless of the form and the cultivation conditions (open and protected ground, in vitro) are characterized by low levels of drought tolerance. Plants growing in open ground conditions demonstrate higher drought tolerance compared with plants in vitro. The biggest water-retaining capacity, both in soil and in vitro conditions, is found in form R100, the lowest – in form 3T.

https://doi.org/10.5281/zenodo.2333352
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References

Hulko, R. (2007), Slovnyk likarskykh roslyn svitovoi medytsyny latynskoiu i shesty slovianskymy movamy. Latynsko-ukrainsko-rosiisko-bolharsko-slovatsko-polsko-cheskyi [The world medical dictionary of medicinal plants in Latin and six Slavic languages. Latin-Ukrainian-Russian-Bulgarian-Slovak-Polish-Czech]. Lviv, Liha-Pres, 443 p.

Dobrenkova, L.H. (1989), Zasukhoustoichyvost sortov zemlianyky ananasnoi v uslovyiakh severo-zapada RSFSR i Krasnodarskoho kraia [Drought resistance of the pineapple strawberry in the conditions of northwest of RSFSR and Krasnodarsk region]. Lviv.: Kataloh myrovoi kollektsyy VYR, vyp. 502, 20 р.

Zavhorodnii, V.M. (2006), Optymizatsiia elementiv tekh nolohii vyroshchuvannia stevii v umovakh Lisostepu Ukrainy [Partial optimization of the Stevia cul tivation technology in the conditions of Ukrainian Forest-Steppe]: Avtoref. dys... kand. s.-h. nauk: 06.01.09. In-t tsukr. buriakiv UAAN. K., 20 p.

http://www.medn.ru/statyi/lekarstvennyie-rasteniya/steviya-stevia-rebaudiana-bertoni.html

Kryshchenko, V.P. (1983), Metody otsenki kachestva rastytelnoi produktsii [The methods of vegetable production quality assessment]. Moskva, Kolos, 192 р.

Metodyka provedennia ekspertyzy sortiv roslyn na vidminnist, odnoridnist ta stabilnist (VOS-TEST). Likarski ta efirooliini, (2014). [Evaluation procedure of different cultivars on diversity, homogeny and stability] Za nauk. red. S.O. Tkachyk. Kyiv, Nilan Ltd, 852 p.

Pleshkov, B.P. (1968), Praktykum po byokhymyy rastenyi [Phytochemistry practicum]. Moskva, Kolos, 184 p.

Shpak, L.M., Liubynskaia, A.V., Rakhmetov, D.B. and Levenko, B.A. (2010), Priamaia regeneratsiia rastenii iz listovykh eksplantov Stevia rebaudiana [Direct regeneration of plants from leaf explants of Stevia rebaudiana] Introduktsiia roslyn, zberezhennia ta zbahachennia bioriznomanittia v botanichnykh sadakh i dendropar kakh: Materialy Mizhnarodnoi naukovoi konferentsii prysviachenoi 75-richchiu zasnuvannia NBS im. M.M. Hryshka NAN Ukrainy, 15—17 veresnia 2010 r. K.: Fitosotsiotsentr, pp. 630—632.

Rohovskyi, S.V. (1992), Razmnozhenie stevii (Stevia rebaudiana Bertoni) cherenkami i osobennosti vy rashchivaniia v usloviiakh Pravoberezhnoi Lesostepi Ukrainy [Propagation by cuttings of stevia (Stevia rebaudiana Bertoni) and peculiarities of its cultivation in conditions of Right-Bank Forest-Steppe of Ukraine]. Avtoref. dys…kand. s.-kh. nauk 06.01.09. In-t sakh. svekly UAAN. Кyiv, 25 p.

Stefaniuk, V.Y. (2009), Steviia v Ukraini [Stevia in Ukraine]. 2-e vyd., dop. Кyiv, Trud-HrynPol, 128 p.

Fitokhimicheskii analiz lekarstvennogo rastitelnogo syria. Metodicheskie ukazaniia k laboratornym zaniatiiam, (1996), [Photochemical analysis of herbal medicinal plants. Methodical guidance to the laboratory practicum]. Sankt-Peterburh, S.-P. hos. khym.-farm. akad.

Shpak, L.M., Rakhmetov, D.B. and Levenko, B.A. (2012), Dlitelnoe khranenie stevii v kulture in vitro [Long term storage of stevia in in vitro culture]. Dendrolohiia, kvitnykarstvo ta sadovo-parkove budivnytstvo: Materialy Mizhnarodnoi naukovoi konferentsii, prysviachenoi 200-richchiu Nikitskoho botanichnoho sadu (5— 8 chervnia 2012 r., Yalta). Yalta, p. 177.

Shpak, L.M., Rakhmetov, D.B. and Levenko, B.A. (2012), Dlitelnoe khranenye Stevia rebaudiana Bert. v kulture in vitro [Long term storage of Stevia rebaudiana Bert. in in vitro culture]. Problemy eksperymentalnoi botaniky ta biotekhnolohii [Problems of experimental botany an biotechnology]. Kyiv: Fitosotsiotsentr, 240 p.

Ahmed, B., Hossain, M., Islam, R. et al. (2011), A review on natural sweetener plant — Stevia having medicinal and commercial importance. Agronomski Glasnik, N 1, 2, pp. 75—91.

Bondarev, N., Reshetnyak, O.V. and Nosov, A.M. (2001), Peculiarities of diterpenoid steviol glycoside production in in vitro cultures of Stevia rebaudiana Bertoni. Plant Science, vol. 161, pp. 155—163.

Tamura, Y., Nakamura, S., Fukui, H., and Tabata, M. (1984), Comparison of Stevia plants grown from seeds, cuttings and stem-tip cultures for growth and sweet diterpene glycosides. Plant Cell Rep., N 3, рр. 180—182.

Lyakhovkin, A.G., Long, T.D., Titov D.A., and Anh, M.P. (1993), Cultivation and utilization of stevia (Stevia rebaudiana Bertoni), Hanoi, Agricultural Publishing House, pp 1—44.

Dictionary of Natural Products, ver.24.2. CRC Press., Taylor & Francis Group, 2016 (Moda access: http://dnp.chemnetbase.com).

Esmat Abou-Arab, A. and Ferial Abu-Salem, M. (2010), Evaluation of bioactive compounds of Stevia rebaudiana leaves and callus. Afr. J. Food Science, vol. 4(10), pp. 627—634.

Esmat Abou-Arab, A., Abou-Arab, A. and Ferial Abu-Salem, M. (2010), Physico-chemical assessment of natural sweeteners steviosides produced from Stevia rebaudiana bertoni plant. Afr. J. Food Science, vol. 4(5), pp. 269 — 281.

Bergs, D., Burghoff, B., Joehnck, M., Martin, G. and Schembecker, G. (2012), Fastandisocratic HPLC method for steviolglycosides analysis from Stevia rebaudiana leaves. J. Verbr. Lebensm., N 7, pр.147—154.

Ferreira, C.M. and Handro, W. (1988), Micropropagation of Stevia rebaudiana through leaf explants from adult plants. Planta Medica, vol. 54, pp. 157— 160.

Kovylyaeva, G.I., Bakaleinik, G.A., Strobykina, I.Yu., Gubskaya, V.I., Sharipova, R.R., Al’fonsov, V.A., Kataev, V.E. and Tolstikov, A.G. (2007), Glycosides from Stevia rebaudiana. Chemistry of Natural Compounds, vol. 43, рp. 81—85.

Harmanjit, Kaur (2011), Chromatographic determination of stevioside in leaf parts of in vitro and in vivo regenerated plants of Stevia rebaudiana. Int. J. Natural Products Research, N 1 (4), рр. 44—48.

Bovanova, L., Brandsteterova, E. and Boxa, S. (1998), HPLC determination of stevioside in plant material and food samples. Zeitschrift für Lebensmittel Untersuchung und Forschung A. Food and Research Technology. A, vol. 207 (5), pp. 352—355.

Ilenko, I. (1990), Microclonal propagation of Stevia in vitro culture. VHIS. Introduction into culture of stevia — a source of low caloric substitute of sugar. Kyiv: VNIS, pp. 74—79.

Uddin, M.S., Chowdhary, M.S., Khan, M.M., Uddin, M.B., Ahmed, R. and Betan, M.A. (2006), In vitro propagation of Stevia rebaudiana Bert in Bangladesh. Afr. J. Biotechnology, N 5, рр. 1238—1240.

Karaköse, H., Jaiswal, R. and Kuhnert, N. (2011), Characterization and quantification of hydroxycinnamate derivatives in Stevia rebaudiana leaves by LC-MSn. J. Agric. Food Chem., vol. 59 (18), pp. 10143— 10150.

Kinghorn, A.D. (2002), Stevia: The genus Stevia (Medicinal and Aromatic Plants — Industrial Profiles), London and New York: Taylor & Francis, 224 p.

Lindley, J., and Moore, T. (1866), The treasury of botany: a popular dictionary of the vegetable kingdom, with which is incorporated a glossary of botanical terms. London, Longmans, Green, and Co, 648 p.

Noordin, N., Rusli, I., Hidayah Sajahan, N., Mohd Nahar, S.M. and Mohd Nahar, S.H. (2012), Micropropagation of Stevia rebaudiana bertoni through temporary immersion bioreactor system. Research and Development Seminar, Bangi (Malaysia), 26—28 Sep., 2012, 8 p.

Murashige, T. and Skoog, P.A. (1963), A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant., vol. 15, pp. 473—479.

Sivaram, L. and Mukundan, U. (2003), In vitro culture stadies of Stevia Rebaudiana. In vitro Cell. Dev. Biol. — Plant, vol. 39, pp. 520—523.

Madan, S., Ahmad, S., Singh, S.N., Kohli, K., Kumar, Y., Singh, R., and Carg, M. (2010), Stevia rebaudiana (Bert.) Bertoni. — A Review. Ind. J. Natural Products and Resources, vol. 1(3), pp. 267—286.

Bondarev, N.І., Sukhanova, M.A., Reshetnyak, O.V. and Nosov A.M. (2003), Steviol glycoside content in different organs of Stevia rebaudiana and its dynamics during ontogeny. Biologia Plantarum, vol. 47, pp. 261—264.

Katayama, O., Sumida, T., Hayashi, H. and Mitsuhashi, H. (1976), The practical application of stevia research and development data ISU Company Japan, pp. 747.

URL: http://www.medn.ru/statyi/lekarstvennyie-rasteniya/steviya-stevia-rebaudiana-bertoni.html

U.S. Food and Drug Administration (FDA) website. Moda access: http://www.fda.gov

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