The effect of nighttime lighting on the anatomical and physiological features of the leaves of linden, horse chestnut, and plane trees in garden-park and street plantings of Kyiv


Tilia cordata
Aesculus hippocastanum
Platanus acerifolia
nighttime lighting
photosynthetic pigments
stomata area
palisade mesophyll

How to Cite

Zaimenko, N., Klymchuk, D., Akimov, Y., Kuchma, T., Didyk, N., Chudovska, O., & Ivanytska, B. (2023). The effect of nighttime lighting on the anatomical and physiological features of the leaves of linden, horse chestnut, and plane trees in garden-park and street plantings of Kyiv. Plant Introduction, (97/98), 33-45.


The effect of nighttime lighting on the anatomical and morphological structure and the content of photosynthetic pigments in the leaves of Tilia cordata, Aesculus hippocastanum, and Platanus acerifolia was estimated on the example of garden-park and street plantings of Kyiv. At the experimental sites, the level of illumination and the soil surface temperature during the day and night periods were examined. The anatomical and morphological structure of the leaves was studied using transmission electron microscopy. The content of photosynthetic pigments (chlorophylls and carotenoids) in tree leaves was determined spectrophotometrically.
The analysis of variance revealed that nighttime lighting significantly affected the anatomical structure and the content of photosynthetic pigments in the leaves of T. cordata and P. acerifolia. In A. hippocastanum, only parameters of stomata and palisade parenchyma showed a significant reaction to this stress factor.



Bian, Z., Yang, Q., Li, T., Cheng, R., Barnett, Y., & Lu, C. (2018). Study of the beneficial effects of green light on lettuce grown under short-term continuous red and blue light-emitting diodes. Physiologia Plantarum, 164(2), 226–240.

Briggs, W.R. (2006). Physiology of plant responses to artificial lighting. In: C. Rich & T. Longcore (Eds.), Ecological consequences of artificial night lighting. Island Press, Washington.

Conley, M., Paparozzi, E. & Stroup, W. (2002). Leaf anatomical and nutrient concentration responses to nitrogen and sulfur applications in poinsettia. Journal of Plant Nutrition, 25, 1773–1791.

Demers, D.A., Dorais, M., Wien, C.H., & Gosselin, A. (1998). Effects of supplemental light duration on greenhouse tomato (Lycopersicon esculentum Mill.) plants and fruit yields. Scientia Horticulturae, 74(4), 295–306.

Dijkshoorn, W., van Broekhoven, L.W., & Lampe, J.E.M. (1979). Phytotoxicity of zinc, nickel, cadmium, lead, copper and chromium in three pasture plant species supplied with graduated amounts from the soil. Netherlands Journal of Agricultural Science, 27(3), 241–253.

Fernández, V., Eichert, T., Río, V., López-Casado, G., Heredia-Guerrero, J., Abadía, A., Heredia, A., & Abadía, J. (2008). Leaf structural changes associated with iron deficiency chlorosis in field-grown pear and peach: physiological implications. Plant and Soil, 311, 161–172.

Gaston, K.J., Bennie, J., Davies, T.W., & Hopkins, J. (2013). The ecological impacts of nighttime light pollution: a mechanistic appraisal. Biological Review, 88(4), 912–927.

Gorelick, N., Hancher, M., Dixon, M., Ilyushchenko, S., Thau, D., & Moore, R. (2017). Google Earth Engine: Planetary-scale geospatial analysis for everyone. Remote Sensing of Environment, 202, 18–27.

Hiscox, J.D., & Israelstam, C.F. (1979). A method for the extraction of chlorophyll from leaf tissue without maceration. Canadian Journal of Botany, 57, 1332–1334.

Ksas, B., Becuwe, N., Chevalier, A., & Havaux, M. (2015). Plant tolerance to excess light energy and photooxidative damage relies on plastoquinone biosynthesis. Scientific Reports, 5, Article 10919.

Kumawat, R.N., Rathore, P.S., Nathawat, N.S., & Mahatma, M. (2006). Effect of sulfur and iron on enzymatic activity and chlorophyll content of mung bean. Journal of Plant Nutrition, 29(8), 1451–1467.

Kwak, M.J., Je, S.M., Cheng, H.C., Seo, S.M., Park, J.H., Baek, S.G., Khaine, I., Lee, T., Jang, J. Li, Y., Kim, H., Lee, J. K., Kim, J., & Woo, S.Y. (2018). Night light-adaptation strategies for photosynthetic apparatus in yellow-poplar (Liriodendron tulipifera L.) exposed to artificial night lighting. Forests, 9(2), Article 74.

Levon, F.M. (1999). Street plantings of Kyiv: current state, ways of optimization. Scientific Bulletin of NAU. Series Forestry, 20, 109–118. (In Ukrainian)

Li, J., Cao, X., Jia, X., Liu, L., Cao, H., Qin, W., & Li, M. (2021). Iron deficiency leads to chlorosis through impacting chlorophyll synthesis and nitrogen metabolism in Areca catechu L. Frontiers in Plant Science, 12, Article 710093.

Liu, X., & Li, Y. (2016). Varietal difference in the correlation between leaf nitrogen content and photosynthesis in rice (Oryza sativa L.) plants is related to specific leaf weight. Journal of Integrative Agriculture, 15(9), 2002–2011.

Meravi, N., & Prajapati, S.K. (2018). Effect street light pollution on the photosynthetic efficiency of different plants. Biological Rhythm Research, 51(1), 67–75.

Miroshnyk, N.V., Tertychna, O.V., & Teslenko, I.K. (2019). Evaluation of ecological threats to park and forest ecosystems. Factors of Experimental Evolution of Organisms, 25, 348–354. (In Ukrainian)

NASA/NOAA Suomi National Polar-orbiting Partnership. (2022). Visible Infrared Imaging Radiometer Suite (VIIRS).

Papish, I. (2001). Practicum on soil physics. Part 2. Soil hydrophysics. Ivan Franko Lviv National University Publishing Center, Lviv. (In Ukrainian)

Raven, J.A., & Cockell, C.S. (2006). Influence on photosynthesis of starlight, moonlight, planetlight and light pollution (reflections on photosynthetically active radiation in the universe). Astrobiology, 6(4), 668–675.

Rinkis, H.I., & Nollendorf, V.F. (1982). Balanced nutrition of plants with macro- and microelements. Zynatne, Riga. (In Russian)

Škvareninová, J., Tuhárska, M., Škvarenina, J., Babálová, D., Slobodníková, L., Slobodník, B., Středová, H, & Minďaš J. (2017). Effects of light pollution on tree phenology in the urban environment. Moravian Geographical Reports, 25(4), 282–290.

Sodani, R., Mishra, U.N., Chand, S., Anuragi, I., Anuragi, H., Chandra, K., Chauhan, J., Bose, B., Kumar, V., Singh, G.S., Lenka, D., & Singhal, R. (2022). Artificial light at night: a global threat to plant biological rhythms and eco-physiological processes. In: L. Hufnagel (Ed.), Light pollution, urbanization and ecology. IntechOpen.

Tewolde, F.T., Lu, N., Shiina, K., Maruo, T., Takagaki, M., Kozai, T., & Yamori, W. (2016). Nighttime supplemental LED inter-lighting improves growth and yield of single-truss tomatoes by enhancing photosynthesis in both winter and summer. Frontiers of Plant Science, 7, Article 448.

Van Gestel, N.C., Nesbit, A.D., Gordon, E.P., Green, C., Paré, P.W., Thompson, L., Peffley, E.B., & Tissue, D.T. (2005). Continuous light may induce photosynthetic downregulation in onion–consequences for growth and biomass partitioning. Physiologia Plantarum, 125, 235–246.

Wellburn, A.R. (1994). The spectral determination of chlorophylls a and b, as well as total carotenoids, using various solvents with spectrophotometers of different resolution. Journal of Plant Physiology, 144(3), 307–313.

Yang, J., & Duan, R. (2019). The effect of artificial illumination on postponing plant phenology. Applied Ecology and Environmental Research, 17(6), 13289–13296.

Yue, C., Wang, Z. & Yang, P. (2021). Review: the effect of light on the key pigment compounds of photosensitive etiolated tea plant. Botanical Studies, 62, Article 21.

Zaimenko, N., Didyk, N., Ellanska, N., Rositska, N., Kharytonova, I., & Yunosheva, O. (2021). Implementation of modern technologies to alleviate soil sickness in urban green areas. Science and Innovation, 17(1), 64–77.

Zhuk, A.V., & Zarochentseva, O.D. (2021). Peculiarities of the environmental light pollution in Chernivtsi region. Ukrainian Hydrometeorological Journal, 27, 66–75. (In Ukrainian).

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