Biochemical and allelopathic features of Adonis vernalis, Allium ursinum, and Leucojum vernum in the M.M. Gryshko National Botanical Garden of the NAS of Ukraine


biogenic elements
phenolic compounds
plant adaptation

How to Cite

ZaіmenkoN., Gnatiuk, A., Gritsenko, V., Zakrasov, O., Pavliuchenko, N., Kharytonova, I., Dziuba, O., Didyk, N., Yunosheva, O., Blum, O., Likhanov, A., & Holichenko, N. (2024). Biochemical and allelopathic features of Adonis vernalis, Allium ursinum, and Leucojum vernum in the M.M. Gryshko National Botanical Garden of the NAS of Ukraine. Plant Introduction, (101/102), 3-18.


The article presents the results of a study on the content and dynamics of the accumulation of biogenic elements and brassinolides in plants of Adonis vernalis, Allium ursinum, and Leucojum vernum in Kyiv, Ukraine. Data is provided on allelopathic activity, content of macro- and microelements, phenolic compounds, and laccase activity in plants and the rhizosphere soil under the conditions of the M.M. Gryshko National Botanical Garden of the National Academy of Sciences of Ukraine (NBG). The plants from the collection of the NBG were used as objects of study in field experiments. The content of biogenic elements in plant tissues and soil was analyzed using an inductively coupled plasma spectrometer. The allelopathic analysis of soil was conducted using a direct bioassay method with Lepidium sativum seedlings as the test object. Phenolic compounds were extracted from the soil using the ion exchange (desorption) method. The content of brassinosteroids was measured spectrophotometrically at a wavelength of 450 nm. The content of laccase was measured spectrophotometrically at a wavelength of 530 nm.
The results demonstrate that model plant species employ a wide range of physiological mechanisms throughout the vegetation period to enhance their resistance to abiotic factors. These mechanisms include maintaining potassium and calcium balance and utilizing hormonal compounds. Plants have been proven to have compensatory mechanisms in response to stress factors, substituting one biochemical marker of resistance with another. Both, brassinosteroids and silicon, contribute to the adaptive capacity of organisms.


Acamovic, T., & Brooker, J.D. (2005). Biochemistry of plant secondary metabolites and their effects in animals. Proceedings of the Nutrition Society, 64(3), 403–412.

Ahire, M.L., Mundada, P.S., Nikam, T.D., Bapat, V.A., & Suprasanna Penna (2021). Multifaceted roles of silicon in mitigating environmental stresses in plants. Plant Physiology and Biochemistry, 169, 291–310.

Alasalvar, C., Grigor, J.M., Zhang, D.L., Quantick, P.C., & Shahidi, F. (2001). Comparison of volatiles, phenolics, sugars, antioxidant vitamins, and sensory quality of different colored carrot varieties. Journal of Agricultural and Food Chemistry, 49(3), 1410–1416.

Babbar, N., Oberoi, H.S., Sandhu, S.K., & Bhargav, V.K. (2014). Influence of different solvents in extraction of phenolic compounds from vegetable residues and their evaluation as natural sources of antioxidants. Journal of Food Science and Technology, 51, 2568–2575.

Baldrian P. (2006). Fungal laccases – occurrence and properties. FEMS Microbiology Reviews, 30(2), 215–242.

Baldrian, P. (2009). Microbial enzyme-catalized processes in soils and their analysis. Plant, Soil and Environment, 55(9), 370–378.

Barillot, C.D.C., Sarde, CO., Bert, V., Tarnaud, E., & Coche, N. (2013). A standardized method for the sampling of rhizosphere and rhizoplan soil bacteria associated to a herbaceous root system. Annals of Microbiology, 63, 471–476.

Basu, S., & Kumar, G. (2021). Exploring the significant contribution of silicon in regulation of cellular redox homeostasis for conferring stress tolerance in plants. Plant Physiology and Biochemistry, 166, 393–404.

Ben Younes, S., Ben Khedher, S., Zhang, Y., Geissen, S.-U., & Sayadi, S. (2019). Laccase from Scytalidium thermophilum: production improvement, catalytic behavior and detoxifying ability of diclofenac. Catalysis Letters, 149, 1833–1844.

Birks, J. (2006). Cholinesterase inhibitors for Alzheimer’s disease. Cochrane Database of Systematic Reviews, 1, Article CD005593.

Chung, S., Kwon, C., & Lee, J.H. (2022). Epigenetic control of abiotic stress signaling in plants. Genes & Genomics, 44(3), 267–278.

Colalto, C. (2020). Safety assessment of homeopathic medicines: the Adonis vernalis paradox and the ‘analysis trap’ of using different pharmacopeias. Journal of Applied Toxicology, 40(11), 1454–1466.

Cui, J., & Tcherkez, G. (2021). Potassium dependency of enzymes in plant primary metabolism. Plant Physiology and Biochemistry, 166, 522–530.

Dhiman, P., Rajora, N., Bhardwaj, S., Sudhakaran, S.S., Kumar, A., Raturi, G., Chakraborty, K., Gupta, O.P., Devanna, B.N., Tripathi, D.K., & Deshmukh, R. (2021). Fascinating role of silicon to combat salinity stress in plants: an updated overview. Plant Physiology and Biochemistry, 162, 110–123.

Didukh, Y.P. (Ed.). (2009). Red book of Ukraine. The plant kingdom. Globalconsulting, Kyiv. (In Ukrainian)

Dong, Q., Bai, B., Almutairi, B.O., & Kudla, J. (2021). Emerging roles of the CBL-CIPK calcium signaling network as key regulatory hub in plant nutrition. Journal of Plant Physiology, 257, Article 153335.

Dragoeva, A.P., Koleva, V.P., Nanova, Z.D., & Georgiev, B.P. (2015). Allelopathic effects of Adonis vernalis L.: root growth inhibition and cytogenetic alterations. Journal of Agricultural Chemistry and Environment, 4(2), Article 56454.

Edreva, A., Velikova, V., Tsonev, T., Dagnon, S., Gürel, A.L., & Aktas, L. (2008). Stress-protective role of secondary metabolites: diversity of functionsand mechanisms. General and Applied Plant Physiology, 34, 67–78.

Eichlerová, I., Šnajdr, J., & Baldrian, P. (2012). Laccase activity in soils: considerations for the measurement of enzyme activity. Chemosphere, 88(10), 1154–1160.

Ellison, S.L.R., & Williams, A. (Eds.). (2012). Eurachem/CITAC guide: quantifying uncertainty in analytical measurement. Third edition. EURACHEM/CITAC.

Etesami, H., & Jeong, B.R. (2023). Chapter 22 – How does silicon help alleviate biotic and abiotic stresses in plants? Mechanisms and future prospects. In M. Ghorbanpour & M.A. Shahid (Eds.), Plant Stress Mitigators (pp. 359–402). Academic Press.

Forgo, P., & Hohmann, J. (2005). Leucovernine and acetylleucovernine, alkaloids from Leucojum vernum. Journal of Natural Products, 68(11), 1588–1591.

Frew, A., Weston, L.A., Reynolds, O.L., & Gurr, G.M. (2018). The role of silicon in plant biology: a paradigm shift in research approach. Annals of Botany, 121(7), 1265–1273.

Ghassemi-Golezani, K., Hassanzadeh, N., Shakiba, M.-R., & Esmaeilpour, B. (2020). Exogenous salicylic acid and 24-epi-brassinolide improve antioxidant capacity and secondary metabolites of Brassica nigra. Biocatalysis and Agricultural Biotechnology, 26, Article 101636.

Ghosh, S., Bheri, M., Bisht, D., & Pandey, G.K. (2022). Calcium signaling and transport machinery: Potential for development of stress tolerance in plants. Current Plant Biology, 29, Article 100235.

Gnatiuk, A.M., & Gaponenko, M.B. (2023, August 3–4). Leucojum vernum L. (Amaryllidaceae) in the collection of the M.M. Gryshko National Botanical Garden of the National Academy of Sciences of Ukraine. In: Materials of the All-Ukrainian Scientific and Practical Conference “Objects of the nature reserve fund of Ukraine: current state and ways to ensure their effective operation” (pp. 270–274). Slavuta. (In Ukrainian).

Golovatskaya, I.F., & Nikonorova, N.M. (2008). Growth and productivity of plants depending on their sensitivity to light and the method of treatment with brassinolide. Agrochemistry, 1, 46–51. (In Russian)

Gritsenko, V.V. (2023). Adonis vernalis L. in the conditions of introduction on the botanical-geographical plot “Steppes of Ukraine” at the M.M. Gryshko National Botanical Garden of the NAS of Ukraine. Journal of Native and Alien Plant Studies, 19, 15–37. (In Ukrainian).

Grodzinsky, A.M. (Ed.). (1987). Experimental allelopathy. Naukova Dumka, Kyiv. (In Russian)

Grodzinsky, A.M. (Ed.). (1989). Medicinal plants: encyclopedic reference. Ukrainian Soviet Encyclopedia, Kyiv. (In Ukrainian)

Grodzinsky, A.M. (1973). Principles of chemical interaction of plants. Naukova Dumka, Kyiv. (In Ukrainian)

Hanelt, P., Hammer, K., & Knupffer, H. (Eds.). (1992). The genus Allium – taxonomic problems and genetic resourses. Proceedings of an International Symposium, held at Gatersleben (1991, 11–13 Juni). IPK, Gatersleben.

Ishfaq, M., Wang, Y., Yan, M., Wang, Z., Wu, L., Li, C., & Li, X. (2022). Physiological essence of magnesium in plants and its widespread deficiency in the farming system of China. Frontiers in Plant Science, 13, Article 802274.

Ivanytska, B.O., & Zaimenko, N.V. (2008). Effects of mineral nutrition’s elements on growth of plants with different ecomorphotypes of Araceae Juss. family. Plant Introduction, 40, 72–77. (In Ukrainian).

Kachalova, O.A., & Dzyuba, O.I. (2014). Antibacterial and antifungal activity of onion (Allium ursinum L.) metabolites. Ukrainian Scientific and Medical Youth Journal, 1, 39–41. (In Ukrainian)

Kolupaev, Y.E., Yastreb, T.O., & Lugovaya, A.A. (2016). The role of jasmonates in plant adaptation to abiotic stressors. Plant Physiology and Genetics, 48(2), 95–111. (In Russian)

Kong, X., Xu, L., & Jamiesonm P. (2020). Plant sense: the rise of calcium channels. Trends in Plant Science, 25(9), 838–841.

Kosakivska, I.V., Shcherbatiuk, М.М., Vasyuk, V.A., & Voytenko, L.V. (2019). Plant hormones under heavy metals stress. Bulletin of Kharkiv National Agrarian University. Series Biology, 3(48), 6–27.

Kovalevsky, A.L. (2011). Biogeochemistry of plants. Ukrainian Publishing House. (In Ukrainian)

Kravets, V.S., Kretinin, S.V., Derevyanchuk, M.V., Drach, S.V., Litvinovskaya, R.P., & Khripach, V.A. (2011). Effect of low temperatures on the level of endogenous brassinosteroids. Reports of the National Academy of Sciences of Ukraine, 8, 155. (In Russian)

Kumari, S., Chhillar, H., Chopra, P., Khanna, R.R., Iqbal, M., & Khan, R. (2021). Potassium: a track to develop salinity tolerant plants. Plant Physiology and Biochemistry, 167, 1011–1023.

Levchyk, N.Y., Dzyuba, O.I., Grishko, V.M., Lyubinska, A.V., Berkov, S., Gnatiuk, A.M., & Mironov, O.L. (2021). Allelopathic activity of biologically active substances of species of natural and cultivated flora of Ukraine and Bulgaria. In A.F. Popov (Ed.), Collection of scientific works “Physico-organic chemistry, pharmacology and pharmaceutical technology of biologically active substances”. Issue 3 (pp. 190–197). KNUTD, Kyiv. (In Ukrainian).

Li, X., He, N., Xu, L., Li, S., & Li, M. (2021). Spatial variation in leaf potassium concentrations and its role in plant adaptation strategies. Ecological Indicators, 130, Article 108063.

Liu, J., Hu, J., Li, Y., Li, G., & Wu, H. (2021). Chapter 10 – Calcium channels and transporters in plants under salinity stress. In S.K. Upadhyay (Ed.), Calcium transport elements in plants (pp. 157–169). Academic Press.

Lotfi, R., Abbasi, A., Kalaji, H.M., Eskandari, I., Sedghieh, V., Khorsandi, H., Sadeghian, N., Yadav, S., & Rastogi, A. (2022). The role of potassium on drought resistance of winter wheat cultivars under cold dryland conditions: Probed by chlorophyll a fluorescence. Plant Physiology and Biochemistry, 182, 45–54.

Mathur, S., Kalaji, H.M., & Jajoo, A. (2016). Investigation of deleterious effects of chromium phytotoxicity and photosynthesis in wheat plant. Photosynthetica, 54(2), 185–192.

McGinnity, P. (2015). Silicon and its role in crop production. PhD Thesis.

Mukhopadhyay, M., & Mondal, T.K. (2015). Effect of zinc and boron on growth and water relations of Camellia sinensis (L.) O. Kuntze cv. T-78. National Academy Science Letters, 38, 283–286.

Nedukha, O.M. (2019). Participation of ions of silicon in adaptation of plants to adverse factors. Bulletin of Kharkiv National Agrarian University, Series Biology, 2(47), 23–38.

Nolan, T.M., Vukasinovic, N., Liu, D., Russinova, E., & Yin, Y. (2020). Brassinosteroids: Multidimensional regulators of plant growth, development, and stress responses. The Plant Cell, 32(2), 295–318.

Rangelov, S., & Nicell, J. (2019). Laccase-catalyzed oxidation of mixed aqueous phenolic substrates at low concentrations. Catalysts, 9(4), Article 368.

Rastogi, A., Yadav, S., Hussain, S., Kataria, S., Hajihashemi, S., Kumari, P., Yang, X., & Brestic, M. (2021). Does silicon really matter for the photosynthetic machinery in plants? Plant Physiology and Biochemistry, 169, 40–48.

Rice, E. (1978). Allelopathy. Mir, Moscow. (In Russian)

Rinkis, G.Y., & Nolendorf, V.F. (1982). Balanced nutrition of plants with macro- and microelements. Knowledge, Riga. (In Russian)

Rivera-Hoyos, C.M., Morales-Álvarez, E.D., Poutou-Piñales, R.A., Pedroza-Rodríguez, A.M., RodrÍguez-Vázquez, R., & Delgado-Boada J.M. (2013). Fungal laccases. Fungal Biology Reviews, 27(3–4), 67–82.

Sattari, R., Khayati, G.R., & Hoshyar, R. (2020). Preparation and physical characterization of Adonis vernalis aqueous leaf extract-mediated green synthesized silver nanoparticles and its toxicity effect on breast cancer cells. Journal of Ultrafine Grained and Nanostructured, 53(2), 183–189.

Schulz, H., Storsberg, J., Schmitt, B., & Keusgen, M. (2003). Bärlauch – Modekraut. Gemüse, 39(6), 14–15.

Shang, X., Miao, X., Yang, F., Wang, C., Li, B., Wang, W., Pan, H., Guo, X., Zhang, Y., & Zhang, J. (2019). The genus Adonis as an important cardiac folk medicine: a review of the ethnobotany, phytochemistry and pharmacology. Frontiers in Pharmacology. Sec. Ethnopharmacology, 10, Article 25.

Sharma, B., Kumawat, K.C., Tiwari, S., Kumar, A., Dar, R.A., Singh, U., Cardinale, M. (2022). Silicon and plant nutrition: dynamics, mechanisms of transport, and role of silicon solubilizer microbiomes in sustainable agriculture. Pedosphere, 33(4), 534–555.

Shirataki, Y., Motohashi, N., Tani, S., Sunaga, K., Sakagami, H., Sato, K., Nakashima, H., Kanamoto, T., Wolfard, K., & Molnar, J. (2001). Antioxidative activity of Allium victorialis L. extracts. International Institute of Anticancer Research, Attiki, 21(5), 3331–3339.

Singh, S., Garg, G., & Rizvi, S.I. (2023). Chapter 9 – Plant polyphenols in balancing the redox state during aging. In K.B. Pandey, & M. Suttajit (Eds.), Plant bioactives as natural panacea against age-induced diseases: nutraceuticals and functional lead compounds for drug development (drug discovery update) (pp. 181–195). Elsevier.

Sobolewska, D., Janeczko, Z., Kisiel, W., Podolak, I., Galanty, A., & Trojanowska, D. (2006). Steroidal glycosides from the underground parts of Allium ursinum L. and their cytostatic and antimicrobial activity. Acta Poloniae Pharmaceutica, 63(3), 219–223.

Sobolewska, D., Podolak, I., & Makowska-Wąs, J. (2015). Allium ursinum: botanical, phytochemical and pharmacological overview. Phytochemistry Reviews: Proceedings of the Phytochemical Society of Europe, 14(1), 81–97.

Straley, G.B., & Utech, F.H. (2002). Leucojum aestivum L. In Flora of North America Editorial Committee (Eds.), Flora of North America. Vol. 26. Magnoliophyta: Liliidae: Liliales and Orchidales. Oxford University Press.

Thurston, C. (1994). The structure and function of fungal laccases. Microbiology, 140(1), 19–26.

Tymochko, I.Y., & Hrynyk, O.M. (2015). Study of the content of macro- and microelements in Allium ursinum L. in different types of forest. Scientific Bulletin of NLTU of Ukraine, 25.5, 110–122. (In Ukrainian).

Tyutyunnyk, S.Y., Rebenkov, S.O., Orlov, O.O., & Dolin, V.V. (2007). Forms of finding aluminum in the background areas of forest-swamp ecosystems. Collection of Scientific Works of the Institute of Environmental Geochemistry of the National Academy of Sciences and the Ministry of Emergency Situations of Ukraine, 15, 115–126. (In Ukrainian)

Verma, S., Negi, N.P., Narwal, P., Kumari, P., Kisku, A.V., Gahlot, P., Mittal, N., & Kumar, D. (2022). Calcium signaling in coordinating plant development, circadian oscillations and environmental stress responses in plants. Environmental and Experimental Botany, 201, Article 104935.

Wang, X., Zhu, B., Jiang, Z., & Wang, S. (2019). Calcium-mediation of jasmonate biosynthesis and signaling in plants. Plant Science, 287, Article 110192.

Wang, Y., Gong, Q., Wu, Y., Huang, F., Ismayil, A., Zhang, D., Li, H., Gu, H., Ludman, M., Fátyol, K., Qi, Y., Yoshioka, K., Hanley-Bowdoin, L., Hong, Y., & Liu, Y. (2021). A calmodulin-binding transcription factor links calcium signaling to antiviral RNAi defense in plants. Cell Host & Microbe, 29(9), 1393–1406.E7.

Wink, M. (2008). Ecological roles of alkaloids. In E. Fattorusso, & O. Taglialatella-Scafati (Eds.), Modern alkaloids (pp. 3–52). Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

Zaimenko, N.V. (2008). Scientific principles of structural and functional design of artificial biogeocenosis in the system soil-plant-soil. Naukova Dumka, Kyiv (In Ukrainian).

Zaimenko, N.V. (Ed.). (2021). Modern methods in allelopathic research. Methodical manual. Lira-K, Kyiv. (In Ukrainian)

Zaimenko, N.V., & Rakhmetov, D.B. (Eds.). (2022). Fundamental and applied aspects of the introduction and preservation of plants in the M.M. Gryshko National Botanical Garden of the NAS of Ukraine. Lira-K, Kyiv. (In Ukrainian)

Zaimenko, N.V., Kharitonova, I.P., & Bondarenko, B.A. (2005). Functional features of the root system of plants under various conditions of mineral nutrition. In Proceedings of the IV International Scientific Conference “Regulation of growth, development and productivity of plants” (p. 86). IOOO Law and Economics, Minsk. (In Russian)

Zaimenko, N.V., Skrypchenko, N.V., Ivanytska, B.O., Venediktova, T.B., Kovalska, N.P., Karpiuk, U.V., Stasiv, T.G., & Liu, D. (2022). Peculiarities of the distribution of assimilates in the organs of Schisandra chinensis plants under different soil and climatic conditions. Biosystems Diversity, 30(4), 423–429.

Zavarzina, A.G., Leontievsky, A.A., Golovleva, L.A., & Trofimov, S.Y. (2004). Biotransformation of soil humic acids by blue laccase of Panus tigrinus 8/18: an in vitro study. Soil Biology and Biochemistry, 36(2), 359–369.

Zhang, F., Zhang, H., Xia, Y., Wang, G., Xu, L., & Shen, Z. (2011). Exogenous application of salicylic acid alleviates Cd-toxicity and reduces hydrogen peroxide accumulation in root apoplasts of Phaseolus aureus and Vicia sativa. Plant Cell Reports, 30, 1475–1483.

Zhao, H., Wu, L., Chai, T., Zhang, Y., Tan J., & Ma, S. (2012). The effects of copper, manganese and zinc on plant growth and elemental accumulation in the manganese-hyperaccumulator Phytolacca americana. Journal of Plant Physiology, 169(13), 1243–1252.

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